Once collected, it permanently unlocks the silver ring, gold ring, metaglio left, and metaglio right, causing them to appear as.. Unlocking yellow sign to unlock yellow sign, you must enter the holy forbidden stage, a hidden area accessible after surviving 14 minutes in the moongolow stage with a standard.
Getting the yellow sign relic will require a bit of skill and some patience.. Unlock the yellow sign in vampire survivors, and access new items.